![]() Based on this study we proposed a biosynthetic scheme for paspaline biosynthesis. This scheme has recently been experimentally validated by reconstitution of the pathway in the heterologous host A. Increased chemical complexity is achieved through enzyme-specific decorations of this core structure through the action of two cytochrome P450 monooxygenases, PaxP, and PaxQ. These additional steps have also been experimentally validated by reconstitution of paxilline biosynthesis in the heterologous host A. Successful amplification was achieved for both species using degenerate primers conC1 and conC2 resulting in putative gene fragments that were similar to the corresponding region of the P. These amplification products were 529-bp for P. crustosum, the paxC-like fragment was extended using primers 2402F1 (specific for the conC1-conC2 product) and degenerate primer PBR2 to produce a contiguous sequence of 2020-bp, which included 813-bp that was similar to the 3' end of paxC, an intergenic region of 865-bp, and 342-bp that was similar to the 3' end of paxP from P. Bibcode: 2007PLoSO.2.728S.Janthinellum a second product of 601-bp was amplified using degenerate primers PPF1 and PPR2 that was similar to a fragment of paxP from P. ![]() ↑ "Protistan Diversity in the Arctic: A Case of Paleoclimate Shaping Modern Biodiversity?".↑ "Diverse molecular data demonstrate that commercially available medicinal leeches are not Hirudo medicinalis.".↑ "Convergent Evolution in the Genetic Basis of Müllerian Mimicry in Heliconius Butterflies.".↑ "A Helicobacter pylori TolC Efflux Pump Confers Resistance to Metronidazole.".↑ "Identification of Mycobacteria by Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry.".↑ "3p-Syndrome Defines a Hearing Loss Locus in 3p25.3.".↑ "Genes to Diseases (G2D) Computational Method to Identify Asthma Candidate Genes.".↑ "Equine Multiple Congenital Ocular Anomalies maps to a 4.9 megabase interval on horse chromosome 6.".↑ "Direct detection of methylation in genomic DNA.".↑ "Microeukaryote Community Patterns along an O2/H2S Gradient in a Supersulfidic Anoxic Fjord (Framvaren, Norway)'Ć.".↑ "MHC class I characterization of Indonesian cynomolgus macaques.".↑ "Frequent Intrapatient Recombination between Human Immunodeficiency Virus Type 1 R5 and X4 Envelopes: Implications for Coreceptor Switch'ñø.".↑ "Severe anaemia is not associated with HIV-1 env gene characteristics in Malawian children.".↑ "Residual Human Immunodeficiency Virus Type 1 Viremia in Some Patients on Antiretroviral Therapy Is Dominated by a Small Number of Invariant Clones Rarely Found in Circulating CD4+ T Cells'Ć.".Citations cover a wide variety of biomedical research areas, including HIV research, biogeography and environmental biology, DNA methylation studies, genetic diseases, clinical microbiology, and evolution research and phylogenetics. In April 2009, CodonCode Aligner had been cited in more than 400 scientific publications. The first beta version of CodonCode Aligner was released in April 2003, followed by the first full version in June 2003. ![]() Features include chromatogram editing, end clipping, and vector trimming, sequence assembly and contig editing, aligning cDNA against genomic templates, sequence alignment and editing, alignment of contigs to each other with ClustalW, MUSCLE, or built-in algorithms, mutation detection, including detection of heterozygous single-nucleotide polymorphism, analysis of heterozygous insertions and deletions, start online BLAST searches, restriction analysis (find and view restriction cut sites), trace sharpening, and support for Phred, Phrap, ClustalW, and MUSCLE. ![]()
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